Measuring Arabidopsis Chromatin Accessibility Using DNase I-Polymerase Chain Reaction and DNase I-Chip Assays
نویسندگان
چکیده
منابع مشابه
Measuring Arabidopsis chromatin accessibility using DNase I-polymerase chain reaction and DNase I-chip assays.
DNA accessibility is an important layer of regulation of DNA-dependent processes. Methods that measure DNA accessibility at local and genome-wide scales have facilitated a rapid increase in the knowledge of chromatin architecture in animal and yeast systems. In contrast, much less is known about chromatin organization in plants. We developed a robust DNase I-polymerase chain reaction (PCR) prot...
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Department of Biology and Zurich-Basel Plant Science Center, Eidgenössisch Technische Hochschule Zurich, CH–8092 Zurich, Switzerland (H.S., W.G., L.H.); Functional Genomics Center Zurich, University of Zurich/ Eidgenössisch Technische Hochschule Zurich, CH–8057 Zurich, Switzerland (W.G.); Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Scie...
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In our previous study, c-fos chromatin accessibility was assayed using DNase I digestion and Southern blot analysis. This low-resolution mapping of c-fos chromatin accessibility demonstrated that serum stimulation of the c-fos enhancer induces a reversible increase in c-fos DNase I sensitivity and suggested that a 5' to 3' gradient of DNase I sensitivity may form downstream from the c-fos enhan...
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The chromatin of the lepidopteran Ephestia kuehniella was digested by micrococcal nuclease, DNase I and S1-nuclease combined with DNase I pretreatment. The resulting DNA fragments were analyzed by gel electrophoresis and compared with the DNA fragments of rat liver nuclei obtained by the same process. Extensive homology was revealed between insect and mammalian chromatin structure. The combined...
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BACKGROUND DNase I is an enzyme which cuts duplex DNA at a rate that depends strongly upon its chromatin environment. In combination with high-throughput sequencing (HTS) technology, it can be used to infer genome-wide landscapes of open chromatin regions. Using this technology, systematic identification of hundreds of thousands of DNase I hypersensitive sites (DHS) per cell type has been possi...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 2013
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.113.220400